1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD38 (FITC-65111, Clone: HIT2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD38 (FITC-65111, Clone: HIT2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD38 (65107-1-Ig, Clone:HB7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug mouse IgG1 isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD38 (65111-1-Ig, Clone:HIT2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD38 (65111-1-Ig, Clone:HIT2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 5 ul APC Anti-Human CD28 (APC-65099, Clone:CD28.2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD28 (APC-65099, Clone:CD28.2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug APC-anti-human CD38 (APC-65111, clone HIT2) (red) or 0.25 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD28 (CL488-65099, Clone:CD28.2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD28 (CL488-65099, Clone:CD28.2) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD38 (CL488-65111, Clone:HIT2), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD38 (CL488-65111, Clone:HIT2), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.